Hormone Preparation, Dosage Calculation, and Injection Techniques for Induced Spawning of Fish

Induced spawning of fish often requires hormone injections. Preparations used for hormone-induced spawning must be mixed properly, and the correct amount must be used to be effective. This publication describes common techniques used to calculate the hormone concentration and dose, prepare hormone solutions, and inject the fish for induced spawning. To calculate the proper dosage, 1) the recommended dose, 2) approximate weight of the brood fish, and 3) desired volume of the injection must be determined. The quantity of hormone to be injected can then be calculated from the weight of each individual brood fish and appropriate injection schedule.